Publication

Comparative genomics of Mortierella elongata and its bacterial endosymbiont Mycoavidus cysteinexigens Public

Jessie Uehling,A. Gryganskyi,K Hameed,Tim Tschaplinski,P. K. Misztal,S. Uw,A. Desiró,N. Pol Vande,Z. Du,Z. Zienkiewicz,K. Zienkiewicz,E. Morin,E. Tisserant,R. Splivallo,M. Hainaut,B. Henrissat,R. Ohn,A. Kuo,J. Yan,A. Lipzen,M. Nolan,K. LaButti,K. Barry,A. H. Goldstein,J. Labbé,C. Schadt,G. Tuskan,I. Grigoriev,F. Martin,R. Vilgalys,Gregory Bonito 2017 January 11 Environmental Microbiology, doi:10.1111/1462-2920.13669

Abstract

Endosymbiosis of bacteria by eukaryotes is a defining feature of cellular evolution. In addition to well known bacterial origins for mitochondria and chloroplasts, multiple origins of bacterial endosymbiosis are known within the cells of diverse animals, plants, and fungi. Early-diverging lineages of terrestrial fungi harbor endosymbiotic bacteria belonging to the Burkholderiaceae. We sequenced the metagenome of the soil-inhabiting fungus Mortierella elongata and assembled the complete circular chromosome of its endosymbiont, Mycoavidus cysteinexigens, which we place within in a lineage of endofungal symbionts that are sister clade to Burkholderia. The M. elongata strain AG77 genome features a core set of primary metabolic pathways for degradation of simple carbohydrates and lipid biosynthesis, while the M. cysteinexigens (AG77) genome is reduced in size and function. Experiments using antibiotics to cure the endobacterium from the host demonstrate that the fungal host metabolism is highly modulated by presence/absence of M. cysteinexigens. Independent comparative phylogenomic analyses of fungal and bacterial genomes are consistent with an ancient origin for M. elongata M. cysteinexigens symbiosis, most likely over 350 million years ago and concomitant with the terrestrialization and diversification of fungi and plants.

Citation

Uehling, J., Gryganskyi, A., Hameed, K., Tschaplinski, T., Misztal, P.K., Wu, S., Desirò, A., Vande Pol, N., Du, Z., Zienkiewicz, A., Zienkiewicz, K., Morin, E., Tisserant, E., Splivallo, R., Hainaut, M., Henrissat, B., Ohm, R., Kuo, A., Yan, J., Lipzen, A., Nolan, M., LaButti, K., Barry, K., Goldstein, A.H., Labbé, J., Schadt, C., Tuskan, G., Grigoriev, I., Martin, F., Vilgalys, R. and Bonito, G. (2017), Comparative genomics of Mortierella elongata and its bacterial endosymbiont Mycoavidus cysteinexigens. Environmental Microbiology. Accepted Author Manuscript. doi:10.1111/1462-2920.13669