Characterization of DWARF14 genes in Populus
Kaijie Zheng,Xiaoping Wang,Deborah A. Weighill,Hao-Bo Guo,Meng Xie,Yongil Yang,Jun Yang,Shucai Wang,Daniel A. Jacobson,Hong Guo,Wellington Muchero,Gerald A. Tuskan,Jin-Gui Chen
2016 February 15 Scientific Reports 6: 21593
Strigolactones are a new class of plant hormones regulating shoot
branching and symbiotic interactions with arbuscular mycorrhizal fungi.
Studies of branching mutants in herbaceous plants have identified
several key genes involved in strigolactone biosynthesis or signaling.
The strigolactone signal is perceived by a member of the α/β-fold
hydrolase superfamily, known as DWARF14 (D14). However, little is known
about D14 genes in the woody perennial plants. Here we report the
identification of D14 homologs in the model woody plant Populus trichocarpa. We showed that there are two D14 homologs in P. trichocarpa,
designated as PtD14a and PtD14b that are over 95% similar at the amino
acid level. Expression analysis indicated that the transcript level of PtD14a is generally more abundant than that of PtD14b. However, only PtD14a was able to complement Arabidopsis d14
mutants, suggesting that PtD14a is the functional D14 ortholog. Amino
acid alignment and structural modeling revealed substitutions of several
highly conserved amino acids in the PtD14b protein including a
phenylalanine near the catalytic triad of D14 proteins. This study lays a
foundation for further characterization of strigolactone pathway and
its functions in the woody perennial plants.
Zheng, K., X. Wang, D. A. Weighill, H.-B. Guo, M. Xie, Y. Yang, J. Yang, S. Wang, D. A. Jacobson, H. Guo, W. Muchero, G. A. Tuskan and J.-G. Chen (2016). "Characterization of DWARF14 Genes in Populus." Scientific Reports 6: 21593.