Strigolactone-Regulated Proteins Revealed by iTRAQ-Based Quantitative Proteomics in Arabidopsis
Zhou Li,Olaf Czarnecki,Kaurna Chourey,Jun Yang,Gerald A. Tuskan,Gregory B. Hurst,Chongle Pan,Jin-Gui Chen
2014 February 24 J. Proteome Res., 2014, 13 (3), pp 1359–1372
Strigolactones (SLs) are a new class of plant hormones. In addition to acting as a key inhibitor of shoot branching, SLs stimulate seed germination of root parasitic plants and promote hyphal branching and root colonization of symbiotic arbuscular mycorrhizal fungi. They also regulate many other aspects of plant growth and development. At the transcription level, SL-regulated genes have been reported. However, nothing is known about the proteome regulated by this new class of plant hormones. A quantitative proteomics approach using an isobaric chemical labeling reagent, iTRAQ, to identify the proteome regulated by SLs in Arabidopsis seedlings is presented. It was found that SLs regulate the expression of about three dozen proteins that have not been previously assigned to SL pathways. These findings provide a new tool to investigate the molecular mechanism of action of SLs.
Zhou Li, Olaf Czarnecki, Karuna Chourey, Jun Yang, Gerald A. Tuskan, Gregory B. Hurst, Chongle Pan, and Jin-Gui Che. Strigolaction-Regulated Proteins Revealed by iTRAQ-Based Quantitative Proteomics in Arabidopsis. Journal of Proteome Research